Apparatus for isothermal titration calorimetry (ITC)
BTU Cottbus - Senftenberg | Published June 28, 2017 - Deadline August 1, 2017
An apparatus for isothermal titration calorimetry (ITC) is to be used. The device is used to investigate the binding strength of two (bio) molecules or between molecules and nanoparticles. The binding strength is an essential factor in the development of assays and provides information for the determination of optimal reaction environments, temperature profiles, concentrations of biomolecules in our assays. By means of the isothermal titration calorimetry, binding affinity and thermodynamic parameters of biomolecules can be measured directly and without marking. Quantitative information on the interaction of, for example, antigenic antibodies, DNA-DNA and aptamer-receptor, as well as energy release in enzymatic reactions are produced. The method characterizes the interaction of biomolecules thermodynamically in detail. The measurement data thus obtained are comparable to those from surface plasmon resonance (SPR) measurements. The ITC, like the SPR, has its limitations and typical artifacts. If one considers that both methods are completely independent techniques, a comparison of the obtained data is of great importance for their validation. In the literature there are only a few examples for such a validation. In bioanalytical applications, the thermodynamic data of the ITC contribute to the understanding of binding mechanisms and the optimization of bioreceptors for specific analytes. Another possibility is the analysis of energy release in enzymatic catalysis. These studies are important not only for the understanding of the reaction mechanisms, but also for the development of enzymatic biosensors, for the analysis of enzyme or substrate concentrations and for the testing of the integrity of immobilized enzymes.